Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD24 Monoclonal Antibody (Catalog # MAB5247) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by APC-conjugated Goat anti-Mouse IgG secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD24 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD24

CD24, also known as heat stable antigen (HSA), is a heavily and variably glycosylated 35‑60 kDa GPI-linked sialoprotein that is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is up‑regulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature human CD24 shares 30% and 42% aa sequence identity with mouse and rat CD24, respectively.

Alternate Names

CD24, CD24A

Entrez Gene IDs

100133941 (Human); 12484 (Mouse)

Gene Symbol

CD24

Additional CD24 Products

Product Documents for Human CD24 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD24 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD24 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5247

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD24 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD24 Antibody

Detection of CD24 in Human Blood Lymphocytes by Flow Cytometry.