ATP6V1D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49254

Novus Biologicals, part of Bio-Techne
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NBP2-49254
NBP2-49254-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP6V1D Antibodies »

Product Summary for ATP6V1D Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VNKAQVKIRAKKDNVAGVTLPVFEHYHEGTDSYELTGLARGGEQLAKLKRNYAKAVELLVELASLQTSFVTLDEAIKIT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP6V1D Antibody

Immunocytochemistry/ Immunofluorescence: ATP6V1D Antibody [NBP2-49254]

Immunocytochemistry/ Immunofluorescence: ATP6V1D Antibody [NBP2-49254]

Immunocytochemistry/Immunofluorescence: ATP6V1D Antibody [NBP2-49254] - Analysis of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254]

Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254]

Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human liver.
Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254]

Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254]

Immunohistochemistry-Paraffin: ATP6V1D Antibody [NBP2-49254] - Staining of human kidney shows strong luminal membranous positivity in proximal tubules.
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Applications for ATP6V1D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V1D

ATP6V1D encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein.

Alternate Names

ATP6M, ATPase, H+ transporting lysosomal, member M, ATPase, H+ transporting, lysosomal (vacuolar proton pump), ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D, H(+)-transporting two-sector ATPase, subunit M, vacuolar ATP synthase subunit D, vacuolar H-ATPase subunit D, vacuolar proton pump D subunit, vacuolar proton pump delta polypeptide, Vacuolar proton pump subunit D, vacuolar proton-ATPase subunit D, VATDV-ATPase D subunit, V-ATPase 28 kDa accessory protein, V-ATPase subunit D, VMA8, V-type proton ATPase subunit D

Gene Symbol

ATP6V1D

Additional ATP6V1D Products

Product Documents for ATP6V1D Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATP6V1D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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