CD59 Antibody (YTH53.1) [Janelia Fluor® 585]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65537JF585
Clone YTH53.1 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Janelia Fluor 585
Antibody Source
Monoclonal Rat IgG2B Clone # YTH53.1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD59 Antibody (YTH53.1) [Janelia Fluor® 585]
Immunogen
Human Peripheral Blood T cells
Localization
Cell membrane; lipid anchor; GPI anchor.
Specificity
NB100-65537 recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for CD59 Antibody (YTH53.1) [Janelia Fluor® 585]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CD59
Alternate Names
CD59, HRF20, MACIF, MIC11, Protectin
Gene Symbol
CD59
Additional CD59 Products
Product Documents for CD59 Antibody (YTH53.1) [Janelia Fluor® 585]
Product Specific Notices for CD59 Antibody (YTH53.1) [Janelia Fluor® 585]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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