Fibulin-3/EFEMP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16277
![Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277]](https://resources.bio-techne.com/images/products/EFEMP1-Antibody-Western-Blot-NBP2-16277-img0001.jpg "Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277]")
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Fibulin-3/EFEMP1. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (89%), Bovine (86%).
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Fibulin-3/EFEMP1 Antibody
![Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277]](https://resources.bio-techne.com/images/products/Fibulin-3-EFEMP1-Antibody-Western-Blot-NBP2-16277-img0004.jpg "Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277]")
Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277]
Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP2-16277]](https://resources.bio-techne.com/images/products/Fibulin-3-EFEMP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16277-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP2-16277]")
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP2-16277]
Immunocytochemistry/Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP2-16277] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EFEMP1 protein stained by EFEMP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NBP2-16277]](https://resources.bio-techne.com/images/products/EFEMP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16277-img0002.jpg "Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NBP2-16277]")
Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NBP2-16277]
Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NBP2-16277] - Human colon carcinoma, using EFEMP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for Fibulin-3/EFEMP1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Fibulin 3
Additional Fibulin 3 Products
Product Documents for Fibulin-3/EFEMP1 Antibody
Product Specific Notices for Fibulin-3/EFEMP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.