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GIT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35536

Novus Biologicals, part of Bio-Techne
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NBP3-35536-100ul
NBP3-35536-20ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 450-510 of human GIT2 (NP_476511.1).

Sequence:
ASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GIT2 Antibody

GIT2 Antibody

Western Blot: GIT2 Antibody [NBP3-35536] -

Western Blot: GIT2 Antibody [NBP3-35536] - Western blot analysis of various lysates using GIT2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for GIT2 Antibody

Application
Recommended Usage

Western Blot

1:200 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GIT2

GIT2 encodes a member of the GIT protein family. GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. This gene undergoes extensive alternative splicing; although ten transcript variants have been described, the full length sequence has been determined for only four variants. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.

Alternate Names

ARF GAP GIT2, ARF GTPase-activating protein GIT2, CAT2, CAT-2, cool-associated, tyrosine phosphorylated protein 2, Cool-interacting tyrosine-phosphorylated protein 2, DKFZp686G01261, G protein-coupled receptor kinase interacting ArfGAP 2, G protein-coupled receptor kinase interactor 2, G protein-coupled receptor kinase-interactor 2, KIAA0148GRK-interacting protein 2, MGC760

Gene Symbol

GIT2

Additional GIT2 Products

Product Documents for GIT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GIT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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