O-GlcNAc Antibody (9H6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59385

Novus Biologicals, part of Bio-Techne
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NBP2-59385
NBP2-59385-25ug
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Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 9H6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all O-GlcNAc Antibodies »

Product Specifications

Immunogen

Synthetic B-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for B-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for O-GlcNAc Antibody (9H6)

Western Blot: O-GlcNAc Antibody (9H6) [NBP2-59385]

Western Blot: O-GlcNAc Antibody (9H6) [NBP2-59385]

Western Blot: O-GlcNAc Antibody (9H6) [NBP2-59385] - Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 (NBP2-59385). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody (NBP2-59385) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6 (NBP2-59385). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody (NBP2-59385) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (9H6) [NBP2-59385] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Applications for O-GlcNAc Antibody (9H6)

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:25

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

PEG purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: O-GlcNAc

Alternate Names

GlcNAc, O-linked N-acetylglucosamine

Additional O-GlcNAc Products

Product Documents for O-GlcNAc Antibody (9H6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Antibody (9H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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