PAAF1 Antibody (OTI1F11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73214

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1F11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PAAF1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PAAF1(NP_079431) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAAF1 Antibody (OTI1F11) - Azide and BSA Free

Western Blot: PAAF1 Antibody (OTI1F11)Azide and BSA Free [NBP2-73214]

Western Blot: PAAF1 Antibody (OTI1F11)Azide and BSA Free [NBP2-73214]

Western Blot: PAAF1 Antibody (OTI1F11) - Azide and BSA Free [NBP2-73214] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAAF1 .
Immunocytochemistry/ Immunofluorescence: PAAF1 Antibody (OTI1F11) - Azide and BSA Free [NBP2-73214]

Immunocytochemistry/ Immunofluorescence: PAAF1 Antibody (OTI1F11) - Azide and BSA Free [NBP2-73214]

Immunocytochemistry/Immunofluorescence: PAAF1 Antibody (OTI1F11) - Azide and BSA Free [NBP2-73214] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PAAF1.
Western Blot: PAAF1 Antibody (OTI1F11)Azide and BSA Free [NBP2-73214]

Western Blot: PAAF1 Antibody (OTI1F11)Azide and BSA Free [NBP2-73214]

Western Blot: PAAF1 Antibody (OTI1F11) - Azide and BSA Free [NBP2-73214] - Analysis of extracts (35ug) from 9 different cell lines by using PAAF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for PAAF1 Antibody (OTI1F11) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:400-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAAF1

PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatorycomplex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotesthe recruitment of 19S regula

Alternate Names

FLJ11848, PAAF, proteasomal ATPase-associated factor 1, Protein G-16, Rpn14, WD repeat domain 71, WD repeat-containing protein 71, WDR71

Gene Symbol

PAAF1

Additional PAAF1 Products

Product Documents for PAAF1 Antibody (OTI1F11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PAAF1 Antibody (OTI1F11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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