Semaphorin 3F Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91480
![Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480]](https://resources.bio-techne.com/images/products/Semaphorin-3F-Antibody-Immunohistochemistry-NBP1-91480-img0003.jpg "Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptide directed towards the N terminal of human Sema3f. Peptide sequence FIHAELIPDSAERNDDKLYFFFRERSAEAPQNPAVYARIGRICLNDDGGH. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Semaphorin 3F Antibody
![Western Blot: Semaphorin 3F Antibody [NBP1-91480]](https://resources.bio-techne.com/images/products/Semaphorin-3F-Antibody-Western-Blot-NBP1-91480-img0005.jpg "Western Blot: Semaphorin 3F Antibody [NBP1-91480]")
Western Blot: Semaphorin 3F Antibody [NBP1-91480]
Western Blot: Semaphorin 3F Antibody [NBP1-91480] - Positive control: Lane1 : No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sema3F Primary, Antibody Dilution: 1 ug/ml Secondary Antibody: Anti-AP Secondry, Antibody Dilution: 1 : 1000.![Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480]](https://resources.bio-techne.com/images/products/Semaphorin-3F-Antibody-Immunohistochemistry-NBP1-91480-img0004.jpg "Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480]")
Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480]
Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480] - Researcher: Dr. Chrisna LeVaillant, School of Anatomy and Human BiologyApplication: IPSpecies+tissue/cell type: 1. 5mL conditioned media from HEK293F cells transfected with 1 : Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP.![Western Blot: Semaphorin 3F Antibody [NBP1-91480]](https://resources.bio-techne.com/images/products/Semaphorin-3F-Antibody-Western-Blot-NBP1-91480-img0002.jpg "Western Blot: Semaphorin 3F Antibody [NBP1-91480]")
Western Blot: Semaphorin 3F Antibody [NBP1-91480]
Western Blot: Semaphorin 3F Antibody [NBP1-91480] - Titration: 1.0 ug/ml Positive Control: Mouse Liver.Applications for Semaphorin 3F Antibody
Application
Recommended Usage
Immunoprecipitation
1:10-1:500
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Semaphorin 3F
Additional Semaphorin 3F Products
Product Documents for Semaphorin 3F Antibody
Product Specific Notices for Semaphorin 3F Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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