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SUV420h1 Antibody [DyLight 650]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97313C

Novus Biologicals, part of Bio-Techne
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NBP1-97313C

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot (Negative)

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human SUV420h1 protein (between residues 450-500) [UniProt Q4FZB7]

Localization

Nucleus. Chromosome. Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUV420h1 Antibody [DyLight 650]

Flow Cytometry: SUV420h1 Antibody [DyLight 650] [NBP1-97313C] - An intracellular stain was performed on SK-MEL-28 cells with SUV420h1 Antibody NBP1-97313C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Applications for SUV420h1 Antibody [DyLight 650]

Application
Recommended Usage

Flow Cytometry

5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SUV420h1

SUV420H1 (Histone-lysine N-methyltransferase 5B, KMT5B) is a histone methyltransferase that specifically di- and trimethlyates lysine 20 of H4 (H4 K20Me3), an epigenetic mark of transcriptional repression. This mark, along with trimethylation of histone 3 lysine 9 (H3 K9me3) and monomethylation of H3 lysine 27 (H3 K27me1), define genomic regions of constitutive heterochromatin that are poor in activated genes. This enzyme is essential for development, with Suv420h1 knockout mice proving lethal perinatally and showing overall growth retardation. The RB1 family of proteins have been shown to interact with, and target, KMT5B to histones, enabling the enzyme to function.

Long Name

Histone-lysine N-methyltransferase KMT5B

Alternate Names

CGI-85, KMT5B, Suv4-20h1

Gene Symbol

KMT5B

Additional SUV420h1 Products

Product Documents for SUV420h1 Antibody [DyLight 650]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SUV420h1 Antibody [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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