Can you provide the HPLC conditions for determining the purity of Imidazoquinoline Resiquimod, R-848?

Thank you for inquiry about the Imidazoquinoline Resiquimod, R-848, Cat No NBP2-26231. Each batch of NBP2-26231 is endotoxin free and equal to or greater than 98% pure by HPLC. Identity is determined by Mass Spec, 1H and 13C NMR .

We are interested in the Catalog NBP2-26231, R848. Is the R848 product water soluble and ready to use to stimulate some cell-line in vitro? Do we need another support product?

The R-848 Imidazoquinoline Resiquimod TLR7 and TLR8 ligand NBP2-26231 is provided solubilized in water at a concentration of 1.0mg/ml. The ligand is ready to use and does not require the purchase of any other support product.

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

Name: Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand
Brand: Novus Biologicals
SKU: NBP2-26231

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26231

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Functional Assay

Product Summary for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

CAS#: 144875-48-9.

Product Specifications

Application Notes

Identity determined by MS, 1H-NMR and 13C-NMR. This product is useful for activation of mouse TLR7 and human TLR7 and TLR8. Stimulation of TLR8 has been achieved with 0.5-5 ug/mL. Use in functional assays reported in scientific literature (PMID 26134251).

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25532693)

Scientific Data Images for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - TLR8 stimulation with R-848 measured by SEAP Expression 293T cells were transfected with pCMV/TLR8 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 2 ug/mL or 5 ug/mL of R-848 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorterAssay kit.

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - R-848 specifically activated the TLR7- or TLR8-depedent NF-kB/SEAP reporter cells in a dose dependent manner.

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - Formula: C17H22N4O2

Formulation, Preparation, and Storage

Purification

>98%, by HPLC.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Reconstitution Instructions

Dissolve in DMSO at a concentration of 10 mg/ml. If the substance is not fully soluble, it can be heated up to 60C to ensure complete solubilization. Also soluble in dichloromethane, 100% or 5% methanol; poorly soluble in acetonitrile or ethyl acetate.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20 °C.

Background: Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand is a low molecular weight synthetic molecule of the Imidazoquinoline family. It has potent immunostimulatory activity. It stimulates antibody secretion, cytokine production, protection from apoptosis and CD80 up-regulation. R-848 has been shown to activate only TLR7 and TLR8. This activation is MyD88 dependent and leads to the induction of the transcription factor NF-kB.

Alternate Names

4-Amino-2-(ethoxymethyl)-a,a-dimethyl-1H-imidazo[4,5-c]quinoline-1-ethanol, Imidazoquinoline Resiquimod, Imidazoquinoline Resiquimod (R-848),TLR7 & TLR8 ligand, Imidazoquinoline Resiquimod R-848, R-848, Resiquimod, S 28463

Additional Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand Products

Product Documents for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

Product Datasheets

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COA

SDS

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Product Specific Notices for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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