ABO, Blood Group A Antigen Antibody (HE-193)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44630
Key Product Details
Species Reactivity
Human
Applications
Agglutination, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # HE-193
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Mixture of erythrocytes of blood group A and glyco protein fraction isolated from the saliva of secretors with blood group A
Reactivity Notes
Others not known.
Localization
Cell Surface
Specificity
This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47869)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for ABO, Blood Group A Antigen Antibody (HE-193)
Immunohistochemistry-Paraffin: ABO, Blood Group A Antigen Antibody (HE-193) [NBP2-44630]
Immunohistochemistry-Paraffin: ABO, Blood Group A Antigen Antibody (HE-193) [NBP2-44630] - Human Colon Carcinoma stained with Blood Group Antigen A Monoclonal Antibody (HE-193)Applications for ABO, Blood Group A Antigen Antibody (HE-193)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-4 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ABO, Blood Group A Antigen
Alternate Names
A transferase, A3GALNT, A3GALT1, ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase), ABO glycosyltransferase, B transferase, B(A) alpha-1,3-galactosyltransferase, EC 2.4.1.37, EC 2.4.1.40, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, GTB, Histo-blood group A transferase, histo-blood group A2 transferase, histo-blood group ABO system transferase, Histo-blood group B transferase, NAGAT
Gene Symbol
ABO
UniProt
Additional ABO, Blood Group A Antigen Products
Product Documents for ABO, Blood Group A Antigen Antibody (HE-193)
Product Specific Notices for ABO, Blood Group A Antigen Antibody (HE-193)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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