alpha Tubulin Antibody (DM1A)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23258
Discontinued Product
NBP3-23258 has been discontinued.
View all alpha Tubulin products.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # DM1A
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for alpha Tubulin Antibody (DM1A)
Immunogen
Clone DM1A was generated from purified chick brain tubulin. The antibody recognizes an epitope within the C-terminal end of alpha Tubulin isoforms. The epitope is highly conserved in alpha Tubulin isoforms from most vertebrate species. [UniProt# P02552]
Specificity
The antibody detects a 55 kDa protein corresponding to the molecular mass of alpha Tubulin on SDS-PAGE immunoblots of human, rat, and mouse cells and tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Applications for alpha Tubulin Antibody (DM1A)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: alpha Tubulin
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).
References
1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921
2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718
Long Name
Tubulin Alpha 1a
Alternate Names
Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1
Gene Symbol
TUBA1A
Additional alpha Tubulin Products
Product Documents for alpha Tubulin Antibody (DM1A)
Product Specific Notices for alpha Tubulin Antibody (DM1A)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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