Cofilin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81866

Novus Biologicals, part of Bio-Techne
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NB100-81866
NB100-81866-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Cofilin Antibodies »

Product Specifications

Immunogen

Peptide sequence around aa. 1~5 (M-A-S-G-V) derived from Human Cofilin.

Specificity

Detects endogenous levels of total Cofilin protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cofilin Antibody

Western Blot: Cofilin Antibody [NB100-81866]

Western Blot: Cofilin Antibody [NB100-81866]

Western Blot: Cofilin Antibody [NB100-81866] - Analysis of extracts from MDA cells using cofilin (Ab-3)

Immunohistochemistry-Paraffin: Cofilin Antibody [NB100-81866] -

Immunohistochemistry-Paraffin: Cofilin Antibody [NB100-81866] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81866 (left) or the same antibody preincubated with blocking peptide(right).

Applications for Cofilin Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cofilin

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

Alternate Names

18 kDa phosphoprotein, CFL, cofilin 1 (non-muscle), Cofilin, non-muscle isoform, cofilin-1, p18

Entrez Gene IDs

1072 (Human); 12631 (Mouse); 29271 (Rat)

Gene Symbol

CFL1

UniProt

P23528 (Human)

Additional Cofilin Products

Product Documents for Cofilin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cofilin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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