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ENPP-2/Autotaxin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40573

Novus Biologicals, part of Bio-Techne
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NBP3-40573

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 778-818 on human Ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) protein (GenBank: EAW91988.1)

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Description

Determinant: C-epitope

Concentration: 0.75-0.95 ug/ul in antibody stabilization buffer

Scientific Data Images for ENPP-2/Autotaxin Antibody

ENPP-2/Autotaxin Antibody

Western Blot: Rabbit ENPP-2/Autotaxin pAb [NBP3-40573] -

Western Blot: Rabbit ENPP-2/Autotaxin pAb [NBP3-40573] - A) Rat lung, B) Rat spleen. 1:500 antibody dilution in Dilobffer. Apparent MW is 100 KDa.
ENPP-2/Autotaxin Antibody

Immunohistochemistry-Paraffin: Rabbit ENPP-2/Autotaxin pAb [NBP3-40573] -

Immunohistochemistry-Paraffin: Rabbit ENPP-2/Autotaxin pAb [NBP3-40573] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Applications for ENPP-2/Autotaxin Antibody

Application
Recommended Usage

Dot Blot

1:10000

ELISA

1:10000

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:250
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENPP-2/Autotaxin

The protein encoded by the ENPP2 gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 2

Alternate Names

ATX, Autotaxin, ENPP2, Lysophosphatidic Acid, NPP2, PDNP2

Gene Symbol

ENPP2

Additional ENPP-2/Autotaxin Products

Product Documents for ENPP-2/Autotaxin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ENPP-2/Autotaxin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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