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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot

Label

Alexa Fluor 532

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2128A

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Human HIST3H3 synthetic peptide

Specificity

Detects human HIST3H3 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for HIST3H3 Antibody (2128A) [Alexa Fluor® 532]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HIST3H3

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The HIST3H3 gene is intronless and encodes a 136 amino acid long, 15 kDA histone H3.1t protein that lacks polyA tails and obtain a palindromic termination element. The HIST3H3 gene is located separately from the other H3 genes on the cluster on chromosome 6p22-p21.3. HIST3H3 participates in PKA signaling, development notch signaling pathway, histone modification, and interferon pathway through interactions with genes HIST1H4B, HIST4H4, HIST1H4A, HIST1H4C, and HIST1H4D. HIST3H3 has been researched regarding its role in immunodeficiency, leukemia, prostatitis, huntington's disease, lupus, multiple sclerosis, laryngeal cancer, and lymphomas.

Long Name

Histone Cluster 3 H3

Alternate Names

H3.4, H3/G, H3/T, H3FT, H3t, Histone 3, H3

Additional HIST3H3 Products

Product Documents for HIST3H3 Antibody (2128A) [Alexa Fluor® 532]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HIST3H3 Antibody (2128A) [Alexa Fluor® 532]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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