HSF1 Antibody (JE44-79)

Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444]

Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: K-562 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: A549 cell lysate
Lane 8: HepG2 cell lysate
Lane 9: LoVo cell lysate

Lysates/proteins at 15 ug/Lane.

Predicted band size: 57 kDa
Observed band size: 75 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: HSF1 Antibody (JE44-79) [NBP3-32444]

Immunohistochemistry: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32444) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (JE44-79) [NBP3-32444]

Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunocytochemistry analysis of HeLa cells labeling HSF1 with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.