Human IL-18 R beta/IL-1 R7 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF118
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-18 R beta/IL-1 R7
Phe20-Arg356
Accession # O95256
Phe20-Arg356
Accession # O95256
Specificity
Detects human IL‑18 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-18 R beta (AcPL), recombinant human (rh) IL-18 R, rhIL-1 R1, rhIL-1 RII, and rhIL-1 Rrp2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-18 R beta/IL-1 R7 Antibody
IFN‑ gamma secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R beta/IL‑1 R7 Antibody.
In the presence of Recombinant Human TNF-a (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-y Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-18 R beta/IL-1 R7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF118). The ND50 is typically 0.3-1.2 µg/mL.Applications for Human IL-18 R beta/IL-1 R7 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera (Catalog # 118-AP)
Sample: Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera (Catalog # 118-AP)
Neutralization
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. Novick, D. et al. (1999) Immunity 10(1):127. The Neutralization Dose (ND50) is typically 0.3‑1.2 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4 and 20 ng/mL Recombinant Human TNF‑ alpha.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R beta/IL-1 R7
IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL‑18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.
References
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
- Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.
Long Name
Interleukin 18 Receptor beta
Alternate Names
AcPL, CD218b, IL-1 R7, IL-18Rb, IL18R beta, IL18RAP
Gene Symbol
IL18RAP
UniProt
Additional IL-18 R beta/IL-1 R7 Products
Product Documents for Human IL-18 R beta/IL-1 R7 Antibody
Product Specific Notices for Human IL-18 R beta/IL-1 R7 Antibody
For research use only
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