Human/Mouse MCPIP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7875
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 604421
Product Specifications
Immunogen
E. coli-derived recombinant human MCPIP1
Asp426-Glu599
Accession # Q5D1E8
Asp426-Glu599
Accession # Q5D1E8
Specificity
Detects human MCPIP1 in direct ELISAs and human and mouse MCPIP1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human MCPIP3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse MCPIP1 Antibody
Detection of Human and Mouse MCPIP1 by Western Blot.
Western blot shows lysates of untreated (-) HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours or 10 µg/mL LPS for 3 hours, respectively. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCPIP1 Monoclonal Antibody (Catalog # MAB7875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCPIP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse MCPIP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Untreated HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line and THP‑1 human acute monocytic leukemia cell line, both treated with LPS
Sample: Untreated HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line and THP‑1 human acute monocytic leukemia cell line, both treated with LPS
Reviewed Applications
Read 2 reviews rated 5 using MAB7875 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCPIP1
Long Name
MCP-1-induced Protein 1
Alternate Names
ZC3H12A
Gene Symbol
ZC3H12A
UniProt
Additional MCPIP1 Products
Product Documents for Human/Mouse MCPIP1 Antibody
Product Specific Notices for Human/Mouse MCPIP1 Antibody
For research use only
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