MAdCAM-1 Antibody (314G8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11286

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Product Datasheet / COA / SDS

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Functional, Immunoassay, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 314G8

Concentration

0.1 mg/ml

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Product Specifications

Immunogen

The monocolonal antibody 314G8 reacts with human mucosal addressin cell adhesion molecules-1 (MAdCAM-1)

Epitope

The monocolonal antibody 314G8 recognizes a site in the N-terminal Ig domain of MAdCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for MAdCAM-1 Antibody (314G8)

Application

Recommended Usage

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

For Immunohistochemistry, Flow Cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of MAdCAM-1 to be inactivated.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MAdCAM-1

The monocolonal antibody 314G8 reacts with human mucosal addressin cell adhesion molecules-1 (MAdCAM-1), a key player in mediating the infiltration of leukocytes into chronically inflamed tissue. MAdCAM-1 is a cell-surface Ig superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain and a short cytoplasmatic domain. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4beta7, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T- and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. Mucosal addressin cell adhesion molecule-1 RNA transcripts are predominantly expressed in the small intestine, mesenteric lymph nodes, colon and spleen; and are very weakly expresssed in human pancreas and brain. The monocolonal antibody 314G8 recognizes a site in the N-terminal Ig domain of MAdCAM-1. The monoclonal antibody 314G8 detects MAdCAM-1 on venules in the spleen and small intestine. MAdCAM-1 is strongly expressed in the synovium of osteoarthritis patients, predominantly on the endothelial lining of blood vessels, but also within the vessel lumen. The monoclonal antibody 314G8 may be useful in diagnosis of inflammation in humans by monitoring the presence and levels of MAdCAM-1.

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Gene Symbol

MADCAM1

Additional MAdCAM-1 Products

Product Documents for MAdCAM-1 Antibody (314G8)

Product Datasheets

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COA

SDS

Product Specific Notices for MAdCAM-1 Antibody (314G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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