Mouse Caspase-11/Caspase-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB86481
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 919916
Product Specifications
Immunogen
E. coli-derived recombinant mouse Caspase-11/Caspase-4
Pro81-Glu266
Accession # P70343
Pro81-Glu266
Accession # P70343
Specificity
Detects mouse Caspase-11/Caspase-4 in direct ELISA.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Caspase-11/Caspase-4 Antibody
Caspase-11/Caspase-4 in Mouse Splenocytes.
Caspase-11/Caspase-4 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse Caspase-11/Caspase-4 Monoclonal Antibody (Catalog # MAB86481) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse Caspase-11/Caspase-4 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes treated with LPS
Sample: Immersion fixed mouse splenocytes treated with LPS
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-11/Caspase-4
Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines. Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen. The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373). Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines. Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS. The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta. Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively.
Alternate Names
CASP11, Caspl, Ich-3
Gene Symbol
CASP4
UniProt
Additional Caspase-11/Caspase-4 Products
Product Documents for Mouse Caspase-11/Caspase-4 Antibody
Product Specific Notices for Mouse Caspase-11/Caspase-4 Antibody
For research use only
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