MSH2 Antibody (10G2-R)

Western Blot: MSH2 Antibody (10G2-R) [NBP3-32605]

Western Blot: MSH2 Antibody (10G2-R) [NBP3-32605] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15 ug/Lane)
Lane 2: HEK-293 cell lysate (15 ug/Lane)
Lane 3: A549 cell lysate (15 ug/Lane)
Lane 4: A431 cell lysate (15 ug/Lane)
Lane 5: HCT 116 cell lysate (15 ug/Lane)
Lane 6: SW480 cell lysate (15 ug/Lane)
Lane 7: PC-3M cell lysate (15 ug/Lane)
Lane 8: LNCaP cell lysate (negative) (15 ug/Lane)
Lane 9: LoVo cell lysate (negative) (15 ug/Lane)
Lane 10: NIH/3T3 cell lysate (15 ug/Lane)
Lane 11: RAW264.7 cell lysate (15 ug/Lane)
Lane 12: PC-12 cell lysate (15 ug/Lane)
Lane 13: Mouse testis tissue lysate (25 ug/Lane)
Lane 14: Rat testis tissue lysate (25 ug/Lane)

Predicted band size: 105 kDa
Observed band size: 105 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MSH2 Antibody (10G2-R) [NBP3-32605]

Immunohistochemistry: MSH2 Antibody (10G2-R) [NBP3-32605] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.