PAWR / PAR4 Antibody (10Q2G9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16523

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16523-100ul
NBP3-16523-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10Q2G9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAWR / PAR4 Antibodies »

Product Summary for PAWR / PAR4 Antibody (10Q2G9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 241-340 of human PAWR / PAR4 (Q96IZ0). YSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVVRERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKTLLKVVGQLTR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAWR / PAR4 Antibody (10Q2G9)

Western Blot: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523]

Western Blot: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523]

Western Blot: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Western blot analysis of extracts of various cell lines, using PAWR / PAR4 Rabbit mAb (NBP3-16523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523]

Immunocytochemistry/ Immunofluorescence: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523]

Immunocytochemistry/Immunofluorescence: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Immunofluorescence analysis of HeLa cells using PAWR / PAR4 Rabbit mAb (NBP3-16523) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PAWR / PAR4 Rabbit mAb (NBP3-16523) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for PAWR / PAR4 Antibody (10Q2G9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAWR / PAR4

The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells. [provided by RefSeq]

Alternate Names

par-4, PAR4prostate apoptosis response protein 4, PRKC apoptosis WT1 regulator protein, PRKC, apoptosis, WT1, regulator, Prostate apoptosis response 4 protein, prostate apoptosis response protein PAR-4, transcriptional repressor PAR4, WT1-interacting protein

Gene Symbol

PAWR

Additional PAWR / PAR4 Products

Product Documents for PAWR / PAR4 Antibody (10Q2G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PAWR / PAR4 Antibody (10Q2G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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