Phospho-Tyrosine Antibody (21)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42911

Novus Biologicals, part of Bio-Techne
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NBP2-42911
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 21

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Phospho-Tyrosine Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to Phospho-Tyrosine

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Phospho-Tyrosine Antibody (21)

Western Blot: Phospho-Tyrosine Antibody (21) [NBP2-42911]

Western Blot: Phospho-Tyrosine Antibody (21) [NBP2-42911]

Western Blot: Phospho-Tyrosine Antibody (21) [NBP2-42911] - Analysis of Un-treated (-) and treated (+), 1mM Pervanadate treatment for 30min. Raji whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Phosphotyrosine antibody.
Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (21) [NBP2-42911]

Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (21) [NBP2-42911]

Immunocytochemistry/Immunofluorescence: Phospho-Tyrosine Antibody (21) [NBP2-42911] - Analysis of Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Phosphotyrosine protein stained by Phosphotyrosine antibody [0021] diluted at 1:50. Red: paxillin, a cytoskeleton marker, stained by Paxillin antibody diluted at 1:100.

Applications for Phospho-Tyrosine Antibody (21)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Phospho-Tyrosine

Phosphotyrosine is the phosphorylated version of the amino acid tyrosine. Phosphorylation of histidine, serine, threonine and tyrosine residues acts as a signalling system to control many cellular signalling pathways. The cellular networks which rely on these signals can be very complex, so phosphorylation often occurs on multiple sites on a given protein.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody (21)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phospho-Tyrosine Antibody (21)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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