SSH1 [p Ser978] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23411
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SSH1 (Ser-978) antibody was generated from a phospho-peptide that included amino acids surrounding serine 978 in human SSH1. This sequence has high homology to the conserved site in rat, mouse, chicken, and zebrafish SSH1L. The sequence also has high homology to the conserved site in SSH2L (Ser-1259). [UniProt# Q8WYL5]
Modification
p Ser978
Specificity
The antibody detects a 150 kDa full length recombinant human SSH1 phosphoprotein and this reactivity is eliminated with lambda phosphatase treatment. The antibody also detects SSH1 on immunoblots of rat PC12 and mouse C2C12 cells that were stimulated with calyculin A.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for SSH1 [p Ser978] Antibody
![SSH1 [p Ser978] Antibody](https://resources.bio-techne.com/images/products/nbp3-23411_rabbit-ssh1-p-ser978-pab-19920231820293.jpg "Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] -")
Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] -
Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] - Western blot of human recombinant SSH1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1 (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1 (Ser-978) (lanes 3, 4, 7, & 8).![SSH1 [p Ser978] Antibody](https://resources.bio-techne.com/images/products/nbp3-23411_rabbit-ssh1-p-ser978-pab-19920231240373.jpg "Immunocytochemistry/Immunofluorescence: SSH1 [p Ser978] Antibody [NBP3-23411] -")
Immunocytochemistry/Immunofluorescence: SSH1 [p Ser978] Antibody [NBP3-23411] -
Immunocytochemistry/Immunofluorescence: SSH1 [p Ser978] Antibody [NBP3-23411] - Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1 (C-term.) and anti-SSH1 (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).Applications for SSH1 [p Ser978] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: SSH1
Alternate Names
EC 3.1.3.16, EC 3.1.3.48, FLJ21928, hSSH-1L, KIAA1298FLJ38102, protein phosphatase Slingshot homolog 1, slingshot 1, slingshot homolog 1 (Drosophila), SSH1L, SSH-1L, SSH-like protein 1
Gene Symbol
SSH1
Additional SSH1 Products
Product Documents for SSH1 [p Ser978] Antibody
Product Specific Notices for SSH1 [p Ser978] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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