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Recombinant Human NME4 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52254

Novus Biologicals, part of Bio-Techne
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NBP2-52254-0.01mg
NBP2-52254-0.05mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 33-187 of Human NME4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 120 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NME4 His Protein

SDS-PAGE: Recombinant Human NME4 His Protein [NBP2-52254]

SDS-PAGE: Recombinant Human NME4 His Protein [NBP2-52254]

SDS-Page: NME4 Recombinant Protein [NBP2-52254] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-52254
Formulation 20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 0.2 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NME4

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM]

Alternate Names

EC 2.7.4.6, NDP kinase, mitochondrial, NDPK-D, NM23D, nm23-H4mitochondrial, non-metastatic cells 4, protein expressed in

Gene Symbol

NME4

Additional NME4 Products

Product Documents for Recombinant Human NME4 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human NME4 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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