Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10137-OX
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived cynomolgus monkey OX40/TNFRSF4 protein
Leu29-Ala216, with an C-terminal 6-His tag
Leu29-Ala216, with an C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu29
Predicted Molecular Mass
21 kDa
SDS-PAGE
40-55 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey OX40/TNFRSF4 His tag (Catalog # 10137-OX) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) binds with an ED50 of 0.25-1.5 ng/mL.
When Recombinant Cynomolgus Monkey OX40/TNFRSF4 His tag (Catalog # 10137-OX) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) binds with an ED50 of 0.25-1.5 ng/mL.
Reviewed Applications
Read 1 review rated 5 using 10137-OX in the following applications:
Scientific Data Images for Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein, CF
Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein Binding Activity
When Recombinant Cynomolgus Monkey OX40/TNFRSF4 His-tag (Catalog # 10137-OX) is immobilized at 0.25 µg/mL, 100 µL/well, Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) binds with an ED50 of 0.25-1.5 ng/mL.Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Cynomolgus Monkey OX40/TNFRSF4 His-tag (Catalog # 10137-OX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-55 kDa.Formulation, Preparation and Storage
10137-OX
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: OX40
References
- Hori, T. (2006) Int. J. Hematol. 83:17.
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- Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
- Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
- Xiao, X. et al. (2008) J. Immunol. 181:3193.
- So, T. and M. Croft (2007) J. Immunol. 179:1427.
- Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
- Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
- Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902.
- Griseri, T. et al. (2010) J. Exp. Med. 207:699.
- Xiao, X. et al. (2012) J. Immunol. 188:892.
- Vu, M.D. et al. (2007) Blood 110:2501.
- Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
Alternate Names
ACT-135, CD134, Ly-70, TNFRSF4, Txgp1, TXGP1L
Gene Symbol
TNFRSF4
UniProt
Additional OX40 Products
Product Documents for Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein, CF
Product Specific Notices for Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein, CF
For research use only
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