Western Blot Data Analysis with Simple Western

Viewing Size-Based Assay Data

Simple Western size-based assay data is processed automatically in Compass for Simple Western™, and you get the results as soon as the run is complete. With Compass, you get two choices to view your data:

Graph View

If you're more familiar with capillary electrophoresis, you can see your results in graph view of the electropherogram. This Cartesian representation of immunoassay data provides quantitative information for signal intensity, which is derived from the area under the curve.

Lane View

Sample data may be displayed by lane in a virtual blot-like image similar to traditional Western blot analysis but with higher quality data. The lanes in this view can be rearranged - no need to manually splice lanes together or rerun samples so that they are adjacent to each other.

Learn About Compass Software

Automated protein normalization Western blot data on Simple Western

Reproducible Protein Quantification

Quantitative results like signal height, area, % area, width, S/N, and baseline values for each immunodetected protein are presented in the results table automatically, which may be exported for further analysis if desired.

Our Simple Western systems give you the detection and quantitation you need, whether you’re looking for absolute or relative quantitation or wanting to simultaneously quantitate high and low protein concentrations. Simple Western assays are fully automated by Jess, Abby, Peggy Sue, or Sally Sue and easily standardized, making your results much more reproducible. There’s no blotting step, so inconsistencies caused by protein transfer are eliminated, which really improves your quantitation too!

Simple Western Protein Size Assay Protein Quantification

A Linear Dynamic Range

Simple Western provides a linear dynamic range assay of approximately 3 orders of magnitude, letting you detect proteins over a wider concentration range, not to mention with more accurate quantitation than traditional Western blot.

p24 Lentivirus titer quantification with Simple Western

Protein MW Separation Ranges

With kits offering protein molecular weight (MW) ranges from 2 kDa to 440 kDa, Simple Western assays support the detection of varying protein sizes, resolving small polypeptides or large target proteins like dystrophin. The Simple Western assay data image shows a panel of proteins run using the 12–230 kDa (left) and a 66–440 kDa (right) Separation Modules.

Additional Resources

Customer Success Stories

Chris LaBreck, Ph.D.
Senior Product Scientist, Cell Signaling Technology (CST)

"Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform."

Miranda Orr, Ph.D.
Postdoctoral Fellow, UT Health Science Center

"The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot."

Return to Simple Western Assays

Simple Western Size-Based Assay Data