What Are Organoids?
An organoid is a miniaturized version of an organ that can be generated in vitro under specific culture conditions from primary tissue, embryonic stem cells (ESCs), or induced pluripotent stem cells (iPSCs). Organoids have similar cellular composition and architecture as their tissues of origin and exhibit functional similarities. They are typically composed of multiple different cell types, which are properly arranged with respect to both each other and the extracellular matrix. Organoids are not only capable of self-organization, they are also capable of self-renewal, and are amenable to long-term cultivation and manipulation. Researchers have developed organoid systems for many different human tissues, including intestine, stomach, colon, liver, lung, heart, pancreas, kidney, prostate, inner ear, brain, and heart, among others. These 3D in vitro model systems have opened new avenues for investigating organ development, studying physiology and disease, screening drugs, and exploring personalized medicine. Bio-Techne offers a wide range of products aimed at promoting robust, reproducible organoid growth. From basement membrane extracts to growth factors and small molecules, we have the reagents that you need for establishing optimal organoid culture conditions, with minimal variability from one experiment to the next.
Tools for Organoid Culture
Cultrex 基底膜提取物
Cultrex 基底膜提取物
• 可提供不同样式符合类器官标准的 Cultrex 基底膜提取物,以促进细胞稳健生长、活力和培养条件一致性
• 我们最新、最先进 Cultrex UltiMatrix RGF 基底膜提取物经过优化,提高培养的细胞球、类器官和干细胞的性能以及一致性
• 低生长因子的配方提供更明确的培养体系
• 质量控制以保证批次间性能的一致性
用于类器官培养的重组蛋白
用于类器官培养的重组蛋白
• 我们提供整套常用于类器官培养的 R&D Systems™ 蛋白产品,包括难以表达和纯化的蛋白,如 R-Spondin-1、Noggin 和 Wnt-3a
• 我们的所有重组蛋白均经过严格测试,以确保高水平的生物活性和批间一致性及低内毒素水平
• R&D Systems 蛋白在类器官相关的科学文献中高频引用
• 从基础研究到临床前应用和临床应用,我们提供研究级、无动物源性 RUO 级和无动物源性 GMP 级细胞因子,以满足每位研究人员的需求
• 可以按折扣价格批量订购我们的重组蛋白,也可提供定制蛋白服务
类器官用小分子
类器官用小分子
• 可提供 Tocris™ 小分子用于3D生长基质组分和类器官长期培养的添加剂
• 我们的目录产品包括 ALK 激酶抑制剂 A 83-01、高选择性 GSK-3 抑制剂 CHIR 99021、强效 ROCK 抑制剂 Y-27632 二盐酸盐、p38 MAPK 抑制剂 SB 202190 等
培养基和培养基补充剂
培养基和培养基补充剂
• 我们提供多种不同形式的基础培养基用于类器官培养,包括含或不含葡萄糖、L-谷氨酰胺、4-羟乙基哌嗪乙磺酸 (HEPES)、非必需氨基酸 (NEAA)、核苷、酚红或丙酮酸钠成分的培养基
• 我们开发并生产支持类器官培养的无血清培养基补充剂
• 我们的目录产品包括经过优化的低温保存培养基,可改善细胞复苏和提高活力
• 我们的新型 ExCellerate iPSC 扩增培养基不含动物组分,并且无需添加生长因子
用于检测谱系特异性标志物的抗体
用于检测谱系特异性标志物的抗体
• 我们的目录产品包括 425,000 多种 R&D Systems™ 和 Novus Biologicals™ 抗体,针对超过 25 个物种
• 我们提供未标记和标记的一抗,能够偶联20 种不同的荧光标记
• 我们的一抗在多达 15 种应用中进行过一种或多种验证
• 提供偶联和非偶联的二抗和一系列同种型对照抗体
• 我们的抗体有多种规格可供选择,包括批量规格和试用规格,确保您可以找到研究所需产品
RNAscope 原位杂交分析
RNAscope 原位杂交分析
• 利用此技术在完整细胞和组织中空间定位和定量特定的靶 mRNA
• 该分析可与免疫组织化学或免疫荧光技术结合,在同一玻片上研究 mRNA 和蛋白共表达
• 使用 RNAscope HiPlex 分析,可以同时检测多达 12 种 RNA 靶标
• 目录产品探针可用于 140 多个物种中超过 23,000 个靶标,也可以定制
• RNAscope 原位杂交 (ISH) 分析跨多个不同研究领域在 2,600 多篇出版物中被引用
Organoid Protocols
Organoid Data
在 Cultrex UltiMatrix RGF 基底膜提取物中培育的人肺类器官。从人肺活检组织分离的成体干细胞包埋在 Cultrex UltiMatrix RGF 基底膜提取物(R&D Systems,货号 BME001-05)中并在培养基中培养 20-60 天。肺类器官能够分化并显示肺各种细胞类型的标志物。A) 将肺类器官用抗细胞角蛋白 5 (KRT5)(绿色;Novus Biologicals,货号 NB110-56916)和抗 p63/TP73L(红色;R&D Systems,货号 AF1916)染色,以可视化基底细胞。B) 将肺类器官用抗平足蛋白 (PDPN)(绿色;Novus Biologicals,货号 NB600-1015)染色,以可视化肺泡 I 型细胞。C, D) 将肺类器官用抗 Muc5ac(绿色;Novus Biologicals,货号 NBP2-15196)染色,以可视化杯状细胞,并用抗 Sox2(红色;R&D Systems,货号 MAB2018)染色。所有样本均用 DAPI 细胞核染色(蓝色;Tocris,货号 5748)复染。
人降结肠类器官。从人降结肠分离的成体干细胞包埋在 Cultrex UltiMatrix RGF 基底膜提取物(R&D Systems,货号 BME001-05)中并在生长培养基中培养 30 天,之后成像。A, B) 将降结肠类器官用嗜铬粒蛋白 A(绿色;R&D Systems,货号 MAB90981)染色,以可视化肠道内分泌细胞,并且用 E-钙黏蛋白(红色;R&D Systems,货号 AF748)和 DAPI(蓝色;R&D Systems,货号 5748)复染。
Cultrex UltiMatrix RGF BME 中所培育人类肠道类器官的视频
使用 Cultrex UltiMatrix RGF 基底膜提取物(R&D Systems,货号 BME001-05)培育的人肠类器官中的 MUC2(红色)表达。图片由 LifeCanvas Technologies 提供。
Literature for Organoid Research
The Organoid Handbook
The Organoid Handbook
This handbook highlights some of the notable publications that have used organoids to investigate questions about development, disease, or drug safety. Additionally, it includes recipes and links to the key reagents that our own in-house scientists have used to establish the culture conditions for different types of organoids.
Optimizing Organoid Culture Conditions Application Note
Optimizing Organoid Culture Conditions Application Note
Establishing and optimizing the appropriate conditions for culturing different types of organoids can be technically challenging. Read this application note to learn about the importance of growth factor bioactivity and reagent consistency for achieving robust, reproducible organoid growth.
Organoids Wall Poster
Organoids Wall Poster
Organoids are emerging as powerful new platforms for exploring human organ development, modeling disease, screening for drug toxicity, and investigating personalized medicine. Request this poster to decorate your lab with some of the visually stunning organoid images that have been generated by cell and gene therapy scientists at Bio-Techne.