A Path to Universal iPSCs for Gene Therapy

Bio-Techne may help create off-the-shelf iPSCs that are universally applicable to more patients and help overcome rejection by the patient's immune system. In this App Note, we leverage Bio-Techne's Gene Editing Tools, Pala Cell Sorter and Single Cell Dispenser, and Simple Western™ technology to screen an iPSC genetic knockout library, including knockdown of targets to suppress the expression of HLA as well as several KAR ligands susceptible to NK cell recognition and attack. Compared to flow cytometry, Simple Western technology could readily detect these intracellular targets and reproducibly quantify their expression relative to wild type. Finally, we used the Simple Western platform to screen for residual Cas9 to ensure the safety of engineered iPSCs.