Is it possible to purchase a smaller version of the PARP/Apoptosis Chemiluminescent Assay Kit?

R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

Is it possible to test partial plates when using the PARP/Apoptosis Chemiluminescent Assay Kit?

The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

PARP/Apoptosis Chemiluminescent Assay Kit

Name: PARP/Apoptosis Chemiluminescent Assay Kit
Brand: R&D Systems
SKU: 4685-096-K
Rating: 5 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # 4685-096-K

(1)

Citations (2)

Product Datasheet / COA

For the measurment of PARP-1 activity in cell extracts

Key Product Details

Features:

Ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits:

Chemiluminescent readout
96 well format
Highly sensitive – detects 0.1 mU PARP ~500 cells
Dynamic range between 0.1 to 10 mU PARP
Requires 10-100 ng extract for detection
Assay Time ~3 hrs

Technical Data
Citations / Reviews (1) / FAQs

Summary for PARP/Apoptosis Chemiluminescent Assay Kit

Why Use the PARP/Apoptosis Chemiluminescent Assay Kit?

This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity. During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.

Product Specifications

Measure PARP-1 activity in cell extracts before and after apoptosis.
For the screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents for PARP/Apoptosis Chemiluminescent Assay Kit

10X Activated DNA
PARP-HSA, 10 mUnits/µl
20 mM NAD
Anti-PAR monoclonal Antibody
Goat anti-mouse IgG-HRP
10 mM Etoposide
20X I-PAR Assay Buffer
PARP PeroxyGlow A
PARP PeroxyGlow™ B
5X Antibody Diluent
Histone-Coated White Strip Well Plate, I-PAR

Product Specifications for PARP/Apoptosis Chemiluminescent Assay Kit

Species

Multi-species

Preparation & Storage

Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

PARP/Apoptosis Chemiluminescent Assay Kit

R&D Systems, part of Bio-Techne | Catalog # 4685-096-K

Key Product Details

Summary for PARP/Apoptosis Chemiluminescent Assay Kit

Why Use the PARP/Apoptosis Chemiluminescent Assay Kit?

Product Specifications

Kit Contents for PARP/Apoptosis Chemiluminescent Assay Kit

Product Specifications for PARP/Apoptosis Chemiluminescent Assay Kit

Preparation & Storage

FAQs for PARP/Apoptosis Chemiluminescent Assay Kit

Product Documents for PARP/Apoptosis Chemiluminescent Assay Kit

Product Datasheets

COA

Certificate of Analysis

CONTACT INFORMATION

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