With what species does this kit work?

This kit is not species specific. It is suitable for enzymes that will be conserved across all mammals and for tissue homogenates.

What PARP isoform is detected in this kit?

This is a universal PARP kit. We expect that all 3 isoforms of PARP will be detected by this kit.

Is the kit capable of measuring PARP hyperactivation (ie. greater than 100% of the PARP positive control)?

With this kit, hyperactivation may be possible since it is a cell free system. It is possible that test molecules increase the binding of PARP to the activated DNA or inhibit its dissociation. We are unable to confirm if PARP may become bound to the DNA and trapped within the replication fork or if this would allow for hyperactivation.

What is the ratio of NAD and activated DNA relative to the concentration of enzyme in the final well?

The ratio of PARP to DNA is 2:1 and NAD is in excess in the final well.

PARP Universal Colorimetric Assay Kit

Name: PARP Universal Colorimetric Assay Kit
Brand: R&D Systems
SKU: 4677-096-K

R&D Systems, part of Bio-Techne | Catalog # 4677-096-K

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Citations (46)

Product Datasheet / COA

For the in vitro screening of candidate PARP-1 inhibitors

Key Product Details

Features:

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits:

Colorimetric readout
96 strip-well format
Sensitive – detects 10 mU PARP /well
Assay Time ~3 hrs

Technical Data
Citations / Reviews / FAQs

Summary for PARP Universal Colorimetric Assay Kit

Why Use the PARP/Apoptosis Colorimetric Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents for PARP Universal Colorimetric Assay Kit

3-Aminobenzamide
PARP-HSA, 10 Units/µl
20X PARP Buffer
10X PARP Cocktail
10X Activated DNA
10X Strep-Diluent
Histone-Coated Plate Natural Strip Well Plate
Strep-HRP
TACS-Sapphire

Product Specifications for PARP Universal Colorimetric Assay Kit

Species

Multi-species

Scientific Data Examples for PARP Universal Colorimetric Assay Kit

	Inhibition of PARP Activity by 3-aminobenzamide. Graphical representation of the colorimetric readout of a PARP standard curve (A) and an inhibition curve for the PARP inhibitor 3-aminobenzamide (provided in the kit). (B) The standard curve is used to translate absorbance values to activity units of PARP.

Preparation & Storage

Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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PARP Universal Colorimetric Assay Kit

R&D Systems, part of Bio-Techne | Catalog # 4677-096-K

Key Product Details

Summary for PARP Universal Colorimetric Assay Kit

Why Use the PARP/Apoptosis Colorimetric Assay Kit?

Product Specifications

Kit Contents for PARP Universal Colorimetric Assay Kit

Product Specifications for PARP Universal Colorimetric Assay Kit

Scientific Data Examples for PARP Universal Colorimetric Assay Kit

Preparation & Storage

Customer Reviews for PARP Universal Colorimetric Assay Kit

FAQs for PARP Universal Colorimetric Assay Kit

Product Documents for PARP Universal Colorimetric Assay Kit

Product Datasheets

COA

Certificate of Analysis

CONTACT INFORMATION

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