ALDH2 Antibody (OTI4H2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70151

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # OTI4H2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ALDH2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ALDH2 (NP_000681) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH2 Antibody (OTI4H2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151]

Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151]

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH2.
Flow Cytometry: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151]

Flow Cytometry: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151]

Flow Cytometry: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ALDH2 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151]

Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151]

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-70151] - Staining of HepG2 cells using anti-ALDH2 mouse monoclonal antibody.

Applications for ALDH2 Antibody (OTI4H2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALDH2

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Long Name

Aldehyde Dehydrogenase 2 Family Member

Alternate Names

ALDHI, ALDM

Gene Symbol

ALDH2

Additional ALDH2 Products

Product Documents for ALDH2 Antibody (OTI4H2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ALDH2 Antibody (OTI4H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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