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APOL3 Antibody (4E5)

Novus Biologicals, part of Bio-Techne | Catalog # H00080833-M01

Novus Biologicals, part of Bio-Techne
Discontinued Product
H00080833-M01 has been discontinued. View all APOL3 products.

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4E5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

APOL3 (NP_663615, 240 a.a. ~ 336 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VEHSYTSSAEAEASRLTATSIDRLKVFKEVMRDITPNLLSLLNNYYEATQTIGSEIRAIRQARARARLPVTTWRISAGSGGQAERTIAGTTRAVSRG

Reactivity Notes

Human. Other species not tested.

Specificity

APOL3 - apolipoprotein L, 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for APOL3 Antibody (4E5)

Western Blot: APOL3 Antibody (4E5) [H00080833-M01]

Western Blot: APOL3 Antibody (4E5) [H00080833-M01]

Western Blot: APOL3 Antibody (4E5) [H00080833-M01] - APOL3 monoclonal antibody (M01), clone 4E5 Analysis of APOL3 expression in Jurkat.
Sandwich ELISA: APOL3 Antibody (4E5) [H00080833-M01] - Detection limit for recombinant GST tagged APOL3 is approximately 0.03ng/ml as a capture antibody.

Applications for APOL3 Antibody (4E5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: APOL3

This gene is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. In addition, expression of this gene is upregulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Six transcript variants encoding three different isoforms have been found for this gene.

Alternate Names

APOLIII, apoL-III, apolipoprotein L, 3, apolipoprotein L3, Apolipoprotein L-III, CG12_1, CG121, CG12-1, TNF-inducible protein CG12-1

Entrez Gene IDs

80833 (Human)

Gene Symbol

APOL3

OMIM

607253 (Human)

Additional APOL3 Products

Product Documents for APOL3 Antibody (4E5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for APOL3 Antibody (4E5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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