B-Raf [p Thr401] Antibody (S05-9B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19547

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19547-100ul
NBP3-19547-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-9B6

Concentration

0.3 mg/ml

View all B-Raf Antibodies »

Product Summary for B-Raf [p Thr401] Antibody (S05-9B6)

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Thr401 of human B-Raf (Uniprot # P15056)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B-Raf [p Thr401] Antibody (S05-9B6)

Western Blot: B-Raf [p Thr401] Antibody (S05-9B6) [NBP3-19547] - Western blot detection of B-Raf in K562, C6, 3T3, Hela cell lysates using NBP3-19547 (1:1000 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.

Applications for B-Raf [p Thr401] Antibody (S05-9B6)

Application
Recommended Usage

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf [p Thr401] Antibody (S05-9B6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B-Raf [p Thr401] Antibody (S05-9B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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