beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81045

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-81045-0.025mg
NBP2-81045-0.2mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BBM.1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all beta 2-Microglobulin Antibodies »

Product Summary for beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free

Immunogen

Molt 4, a human T cell line.

Specificity

Binds human beta 2-Microglobulin, a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells) and involved in the presentation of peptide antigens to the immune system.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free

Western Blot: beta 2-Microglobulin Antibody (BBM.1)ChimericAzide and BSA Free [NBP2-81045]

Western Blot: beta 2-Microglobulin Antibody (BBM.1)ChimericAzide and BSA Free [NBP2-81045]

Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045]

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045]

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Immunofluoresence staining of fixed HeLa cells with anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of BBM.1 [NBP2-81045] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81045, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.
Flow Cytometry: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045]

Flow Cytometry: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045]

Flow Cytometry: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (NBP2-81045, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Applications for beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: beta 2-Microglobulin

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.