BHMT Antibody (9E1-R)

Western Blot: BHMT Antibody (9E1-R) [NBP3-32082]

Western Blot: BHMT Antibody (9E1-R) [NBP3-32082] - Western blot analysis of BHMT on different lysates with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution.

Lane 1: Human kidney tissue lysate
Lane 2: Human liver tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32082) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: BHMT Antibody (9E1-R) [NBP3-32082]

Immunohistochemistry: BHMT Antibody (9E1-R) [NBP3-32082] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32082) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.