Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48422
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted [NBP2-48422]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-BRD-3-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48422-img0001.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted [NBP2-48422]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Non-species specific
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BRD.3
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted
Immunogen
Bromodeoxyuridine/BrdU conjugated to BSA
Localization
Nuclear
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted [NBP2-48422]
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted [NBP2-48422] - Mouse Small Intestine stained with BrdU Monoclonal Antibody (BRD.3).Applications for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Bromodeoxyuridine/BrdU
Alternate Names
5-bromo-2-deoxyuridine, BrdU
Additional Bromodeoxyuridine/BrdU Products
Product Documents for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted
Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BRD.3) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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