Carbonic Anhydrase XIV/CA14 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87477

Novus Biologicals, part of Bio-Techne
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NBP1-87477
NBP1-87477-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Carbonic Anhydrase XIV/CA14 Antibodies »

Product Summary for Carbonic Anhydrase XIV/CA14 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GQHWTYEGPHGQDHWPASYPECGNNAQSPIDIQTDSVTFDPDLPALQPHGYDQPGTEPLDLHNNGHTVQLSLPSTLYLGGLPRKYVAAQLHLHWGQKGSPGGSEHQINSEATFAELHIVHYDSDSYDSLSEAAERPQGLAVLGIL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbonic Anhydrase XIV/CA14 Antibody

Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Analysis in control (vector only transfected HEK293T lysate) and CA14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Staining of human cell line SK-MEL-30 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Applications for Carbonic Anhydrase XIV/CA14 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XIV/CA14

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq]

Alternate Names

CA14

Gene Symbol

CA14

Additional Carbonic Anhydrase XIV/CA14 Products

Product Documents for Carbonic Anhydrase XIV/CA14 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase XIV/CA14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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