Catenin alpha 1 [p Tyr148] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23196
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phospho-Catenin alpha 1 (Tyr-148) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 148 in human Catenin alpha 1. This peptide sequence is highly conserved in rat and mouse Catenin alpha 1, but is not conserved in Catenin alpha 2 or Catenin alpha 3. [UniProt# P35221]
Modification
p Tyr148
Specificity
This antibody was cross-adsorbed to unrelated phospho-tyrosine peptide before affinity purification using phospho-Catenin alpha 1 (Tyr-148) peptide (without carrier). The antibody detects a 102 kDa protein corresponding to the molecular mass of a1-Catenin on SDS-PAGE immunoblots of rat PC12 cells treated with pervanadate.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Catenin alpha 1 [p Tyr148] Antibody
![Catenin alpha 1 [p Tyr148] Antibody](https://resources.bio-techne.com/images/products/nbp3-23196_rabbit-catenin-alpha-1-p-tyr148-pab-1992023185692.jpg "Western Blot: Catenin alpha 1 [p Tyr148] Antibody [NBP3-23196] -")
Western Blot: Catenin alpha 1 [p Tyr148] Antibody [NBP3-23196] -
Western Blot: Catenin alpha 1 [p Tyr148] Antibody [NBP3-23196] - Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti- -Catenin monoclonal (lanes 1 & 2), anti-Catenin alpha 1 (Tyr-148) phospho-specific (lanes 3 & 4), or anti-Catenin alpha 1 (a.a. 143-153) (lanes 5 & 6).Applications for Catenin alpha 1 [p Tyr148] Antibody
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Catenin alpha 1
Alternate Names
Alpha E-catenin, alpha-catenin, alpha-E-catenin, alphaE-catenin, Cadherin-associated protein, CAP102, catenin (cadherin-associated protein), alpha 1 (102kD), catenin (cadherin-associated protein), alpha 1, 102kDa, catenin alpha-1, FLJ36832, FLJ52416, Renal carcinoma antigen NY-REN-13
Gene Symbol
CTNNA1
Additional Catenin alpha 1 Products
Product Documents for Catenin alpha 1 [p Tyr148] Antibody
Product Specific Notices for Catenin alpha 1 [p Tyr148] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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