CCL8/MCP-2 Antibody (35509.11)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-10391
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 35509.11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant human monocyte chemotactic protein 2 (MCP2), expressed in E. coli.
Localization
Secreted
Specificity
By ELISA, this antibody shows cross-reactivity with recombinant human MCP1 and recombinat mouse MIP1 alpha. In addition, it does not cross-react with other chemokines tested: rmC10, rhENA78, rhGRO alpha, rhGRO beta, rhGRO gamma, rmJE, rhI309, and rhIL8. This antibody neutralizes the biological activity of recombinant human MCP-2. Shows
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CCL8/MCP-2 Antibody (35509.11)
![Immunocytochemistry/ Immunofluorescence: CCL8/MCP-2 Antibody (35509.11) [NB120-10391]](https://resources.bio-techne.com/images/products/CCL8-MCP-2-Antibody-35509-11-Immunocytochemistry-Immunofluorescence-NB120-10391-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CCL8/MCP-2 Antibody (35509.11) [NB120-10391]")
Immunocytochemistry/ Immunofluorescence: CCL8/MCP-2 Antibody (35509.11) [NB120-10391]
Immunocytochemistry/Immunofluorescence: CCL8/MCP-2 Antibody (35509.11) [NB120-10391] - Panel shows intracellular staining to measure CCL8/MCP-2 levels in untreated or treated MDDCs. Panel shows representative single cell images. Analysis using FITC conjugated version of antibody (NB120-10391F). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28894190/) licensed under a CC-BY license.Applications for CCL8/MCP-2 Antibody (35509.11)
Application
Recommended Usage
ELISA
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1:500-1:1000
Western Blot
1 - 2 ug/ml
Neutralization
1 - 4 ug/ml
Application Notes
ELISA: Use at a concentration of 0.5 - 1 ug/ml to detect 12.5ng/ml of recombinant human MCP2.Neut: Use at a concentration of 0.5 - 10 ug/ml. WB: Use at a concentration of 1 - 2 ug/ml to detect 50ng/lane of recombinant human MCP2 under reducing and non-reducing conditions. Predicted molecular weight: 9 kDa. Human MCP2 is tested for its ability to neutralize the chemotactic activity of rhMCP2 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP2 that is present at a concentration just high enough to elicit a maximum response. In this bioassay 0.4ug/ml rhMCP2 was mixed with various dilutions of the antibody for 30 minutes at 22.
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 28894190).
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 28894190).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Lyophilized from a 0.2 um filtered PBS and 5% Trehalose
Preservative
5% Trehalose
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CCL8/MCP-2
Additional CCL8/MCP-2 Products
Product Documents for CCL8/MCP-2 Antibody (35509.11)
Product Specific Notices for CCL8/MCP-2 Antibody (35509.11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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