CDX2 Antibody (CL12974)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18552

Novus Biologicals, part of Bio-Techne
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NBP3-18552-100ul
NBP3-18552-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12974

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDX2 Antibodies »

Product Summary for CDX2 Antibody (CL12974)

Immunogen

This antibody was developed against a sythetic peptide for Q99626. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CDX2 Antibody (CL12974)

Western Blot: CDX2 Antibody (CL12974) [NBP3-18552]

Western Blot: CDX2 Antibody (CL12974) [NBP3-18552]

Western Blot: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human cell line CACO-2 and human cell line PC-3.
Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (CL12974) [NBP3-18552]

Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (CL12974) [NBP3-18552]

Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of CACO-2 cells using the Anti-CDX2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule is visualized in red (where available).
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
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Applications for CDX2 Antibody (CL12974)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDX2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Gene Symbol

CDX2

Additional CDX2 Products

Product Documents for CDX2 Antibody (CL12974)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDX2 Antibody (CL12974)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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