Claudin-18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-60010

Novus Biologicals, part of Bio-Techne
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NBP1-60010
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Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Claudin-18 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to CLDN18(claudin 18) The peptide sequence was selected from the C terminal of CLDN18. Peptide sequence PEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDE. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Canine reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Claudin-18 Antibody

Western Blot: Claudin-18 Antibody [NBP1-60010]

Western Blot: Claudin-18 Antibody [NBP1-60010]

Western Blot: Claudin-18 Antibody [NBP1-60010] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12
Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP1-60010]

Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP1-60010]

Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP1-60010] - Claudin-18 antibody used at 1:50 concentration in casein in PBS and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in Citrate buffer, pH6. Image from verified customer review.
Western Blot: Claudin-18 Antibody [NBP1-60010]

Western Blot: Claudin-18 Antibody [NBP1-60010]

Western Blot: Claudin-18 Antibody [NBP1-60010] - Tissue:Jurkat cells. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide.
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Applications for Claudin-18 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-60010 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-18

CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells.CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells (Niimi et al., 2001).[supplied by OMIM].

Alternate Names

Claudin18, CLDN18, SFTA5, SFTPJ

Gene Symbol

CLDN18

UniProt

P56856 (Human)

Additional Claudin-18 Products

Product Documents for Claudin-18 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Claudin-18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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