Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CSN1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 400 and the C-terminus (residue 471) of human G protein Pathway Suppressor 1 using the numbering given in Swiss-Prot entry Q13098 (GeneID 2873).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CSN1 Antibody

Western Blot: CSN1 Antibody [NB100-362]

Western Blot: CSN1 Antibody [NB100-362]

Western Blot: CSN1 Antibody [NB100-362] - Detection of Human CSN1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN1 antibody NB100-362 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: CSN1 Antibody [NB100-362]

Immunohistochemistry: CSN1 Antibody [NB100-362]

Immunohistochemistry: CSN1 Antibody [NB100-362] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CSN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: CSN1 Antibody [NB100-362]

Western Blot: CSN1 Antibody [NB100-362]

Western Blot: CSN1 Antibody [NB100-362] - Whole cell lysate (50 ug - E; 10 ug - T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells. Antibody used at the indicated concentration.
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Applications for CSN1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500- 1:2000

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CSN1

COP9 signalosome is a conserved protein composed of eight subunits. Individual subunits of the complex have been linked to various signal transduction pathways leading to gene expression and cell cycle control. CSN1 is the COP9 subunit 1. The C-terminus of CSN1 is responsible for interacting with CSN2, CSN3, and CSN4 subunits. It is required for incorporation of the subunit into the complex. The N-terminus harbors the activity domain that confers most of the repression functions of CSN1.

Alternate Names

COP9 signalosome complex subunit 1, COPS1JAB1-containing signalosome subunit 1, CSN1MGC71287, G protein pathway suppressor 1SGN1, GPS-1, Protein MFH, Signalosome subunit 1

Entrez Gene IDs

2873 (Human)

Gene Symbol

GPS1

UniProt

Q13098 (Human)

Additional CSN1 Products

Product Documents for CSN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CSN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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