Cytochrome c Antibody (SC59-01)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67558

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC59-01

Concentration

1 mg/ml

View all Cytochrome c Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human Cytochrome c aa 10-50. (SwissProt: P99999 Human; SwissProt: P62897 Mouse; SwissProt: P62898 Rat)

Localization

Mitochondrion intermembrane space.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytochrome c Antibody (SC59-01)

Western Blot: Cytochrome c Antibody (SC59-01) [NBP2-67558]

Western Blot: Cytochrome c Antibody (SC59-01) [NBP2-67558]

Western Blot: Cytochrome c Antibody (SC59-01) [NBP2-67558] - Analysis of Cytochrome C on different lysates using anti-Cytochrome C antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse kidney Lane 2: Rat kidney
Immunohistochemistry-Paraffin: Cytochrome c Antibody (SC59-01) [NBP2-67558]

Immunohistochemistry-Paraffin: Cytochrome c Antibody (SC59-01) [NBP2-67558]

Immunohistochemistry-Paraffin: Cytochrome c Antibody (SC59-01) [NBP2-67558] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cytochrome c Antibody (SC59-01) [NBP2-67558]

Immunohistochemistry-Paraffin: Cytochrome c Antibody (SC59-01) [NBP2-67558]

Immunohistochemistry-Paraffin: Cytochrome c Antibody (SC59-01) [NBP2-67558] - Staining Cytochrome C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Applications for Cytochrome c Antibody (SC59-01)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome c

In addition to its well-known role in electron-transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death (1). Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade bay activating caspase-3 (2). Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3 (3).

Alternate Names

CYCS

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents for Cytochrome c Antibody (SC59-01)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytochrome c Antibody (SC59-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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