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Cytokeratin 18 Antibody (DE-K18) [DyLight 594]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11516DL594

Novus Biologicals, part of Bio-Techne
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NBP3-11516DL594

Key Product Details

Species Reactivity

Validated:

Human, Canine, Feline, Rat (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 594 (Excitation = 593 nm, Emission = 618 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DE-K18

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Cytokeratin 18 Antibody (DE-K18) [DyLight 594]

Immunogen

Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Reactivity Notes

Does not react with Rat.

Localization

Cytoplasmic

Specificity

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Cytokeratin 18 Antibody (DE-K18) [DyLight 594]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (DE-K18) [DyLight 594]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 18 Antibody (DE-K18) [DyLight 594]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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