Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 38C674.2

Concentration

1.0 mg/ml

View all Delta Np73 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a peptide corresponding to a portion of amino acid residues 1-50 of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Delta Np73 Antibody (38C674.2)

Western Blot: Delta Np73 Antibody (38C674.2) [NBP2-24873]

Western Blot: Delta Np73 Antibody (38C674.2) [NBP2-24873]

Western Blot: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) analysis for Np73 using NBP2-24873 at 1 ug/ml in A) a cell line transfected with Np73 cDNA, B) HeLa, and C) NIH 3T3 cell lysate.
Immunocytochemistry/ Immunofluorescence: Delta Np73 Antibody (38C674.2) [NBP2-24873]

Immunocytochemistry/ Immunofluorescence: Delta Np73 Antibody (38C674.2) [NBP2-24873]

Immunocytochemistry/Immunofluorescence: Delta Np73 Antibody (38C674.2) [NBP2-24873] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Delta Np73 (38C674.2) NBP2-24873 at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) [NBP2-24873]

Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) [NBP2-24873]

Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Applications for Delta Np73 Antibody (38C674.2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:1000

Immunohistochemistry-Frozen

1:20-1:1000

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

reported in scientific literature (PMID 19625514)

Western Blot

1-3 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Delta Np73

Alternate Names

p53-related protein, p73, tumor protein p73

Gene Symbol

TP73

UniProt

O15350 (Human)

Additional Delta Np73 Products

Product Documents for Delta Np73 Antibody (38C674.2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Delta Np73 Antibody (38C674.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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