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DHX16 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13920

Novus Biologicals, part of Bio-Techne
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NBP2-13920
NBP2-13920-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for DHX16 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: HMPKETRGQPARAVDLVEEESGAPGEEQRRWEEARLGAASLKFGARDAASQEPKYQLVLEEEETIEFVRATQLQGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX16 Antibody

Immunocytochemistry/ Immunofluorescence: DHX16 Antibody [NBP2-13920]

Immunocytochemistry/ Immunofluorescence: DHX16 Antibody [NBP2-13920]

Immunocytochemistry/Immunofluorescence: DHX16 Antibody [NBP2-13920] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920]

Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920]

Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920] - Staining in human testis and liver tissues using anti-DHX16 antibody. Corresponding DHX16 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920]

Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920]

Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920] - Staining of human kidney shows strong nuclear positivity in renal tubules and glomeruli.

Applications for DHX16 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunofluorescence, Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHX16

DHX16, also known as Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16, is a 119.3 kDa 1,041 amino acid protein and is engaged in pre-mRNA splicing and unwinding RNA as a part of the DEAD box protein family. Current research is being conducted with the protein involving diseases such as adenocarcinoma, ataxia, esophagitis, erythematosus, and malaria. The protein interacts in mRNA processing and spliceosome pathways with other proteins such as GPKOW, SF3B2, COIL, DHX38, and MEGF10.

Alternate Names

ATP-dependent RNA helicase #3, DBP2PRO2014, DDX16DEAD/H box 16, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 16, DEAH (Asp-Glu-Ala-His) box polypeptide 16, DEAH-box protein 16, EC 3.6.1, EC 3.6.4.13, KIAA0577, Prp2, PRP8, PRPF2, putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16, RNA helicase

Gene Symbol

DHX16

Additional DHX16 Products

Product Documents for DHX16 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DHX16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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