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Drosha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55410

Novus Biologicals, part of Bio-Techne
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NB100-55410

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KQTDKQKLAQRE corresponding to internal region according to NP_081075.2.

Epitope

KQTDKQKLAQRE

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21931811).

Specificity

This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Drosha Antibody

Western Blot: Drosha Antibody [NB100-55410]

Western Blot: Drosha Antibody [NB100-55410]

Western Blot: Drosha Antibody [NB100-55410] - Staining of Human Liver lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Approx. 150-160 kDa band observed in human liver lysates (calculated MW of 151 kDa band according to NP_081075.2).

Applications for Drosha Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Drosha

Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003).[supplied by OMIM]

Alternate Names

drosha, double-stranded RNA-specific endoribonuclease, drosha, ribonuclease type III, EC 3.1.26.3, ETOHI2, nuclear RNase III Drosha, p241, Protein Drosha, putative protein p241 which interacts with transcription factor Sp1, putative ribonuclease III, RANSE3L, ribonuclease 3, Ribonuclease III, ribonuclease III, nuclear, RN3nuclear, RNase III

Entrez Gene IDs

29102 (Human); 14000 (Mouse); 310159 (Rat)

Gene Symbol

DROSHA

Additional Drosha Products

Product Documents for Drosha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Drosha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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