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eIF2B4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49848

Novus Biologicals, part of Bio-Techne
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NBP1-49848

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-RKDYGSKVSLFSH corresponding to internal region according to NP_001029288.1, NP_056451.3.

Epitope

RKDYGSKVSLFSH - internal region

Specificity

This antibody is expected to recognize all reported isoforms (NP_751945.2; NP_001029288.1; NP_056451.3).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for eIF2B4 Antibody

Western Blot: eIF2B4 Antibody [NBP1-49848]

Western Blot: eIF2B4 Antibody [NBP1-49848]

Western Blot: eIF2B4 Antibody [NBP1-49848] - Analysis of eIF2B4 in K562 lysate (35ug protein in RIPA buffer) using NBP1-49848 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for eIF2B4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in K562 lysates (calculated MW of 59.6 kDa band according to NP_751945.2). An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF2B4

EIF2B4 - eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa

Long Name

Translation initiation factor eIF-2B subunit delta

Alternate Names

DKFZP586J0119, EIF2BD, EIF2Bdelta

Entrez Gene IDs

8890 (Human); 13667 (Mouse); 117019 (Rat)

Gene Symbol

EIF2B4

Additional eIF2B4 Products

Product Documents for eIF2B4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF2B4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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