eIF4B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33284

Novus Biologicals, part of Bio-Techne
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NBP2-33284
NBP2-33284-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF4B Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF4B Antibody

Western Blot: eIF4B Antibody [NBP2-33284]

Western Blot: eIF4B Antibody [NBP2-33284]

Western Blot: eIF4B Antibody [NBP2-33284] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: eIF4B Antibody [NBP2-33284]

Immunocytochemistry/ Immunofluorescence: eIF4B Antibody [NBP2-33284]

Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-33284] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284]

Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284]

Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Applications for eIF4B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4B

Eukaryotic initiation factor 4B (eIF4B) is a factor required for the binding of mRNA to ribosomes for the initiation of translation. eIF4B has been demonstrated to modulate the helicase activity of eIF4A, a component of the eIF4F complex

Long Name

Eukaryotic Translation Initiation Factor 4B

Alternate Names

Eif4a2

Gene Symbol

EIF4B

UniProt

P23588

Additional eIF4B Products

Product Documents for eIF4B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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